Bieber sang "Purpose" as the names of the 49 people who lost their lives during the hateful attack scrolled upward on a screen behind him.
At the end, Christina Grimmie's name appeared. The former Voice contestant was shot and killed in Orlando just a day before the tragic nightclub massacre.
In his Winnepeg show, Bieber had called out to Grimmie while lying on the floor during a performance of "Purpose."
Many celebrities are using their platform to honor the Orlando victims by either saying their name or speaking out against gun violence.
In a video for the Human Rights Campaign, celebrities like Lady Gaga, Cuba Gooding Jr., Jane Fonda, George Lopez, Kerry Washington, Chris Pine and so many others eulogized those killed.
Lady Gaga has been very vocal about the hate against the LGBT community, and attended a vigil in California to take part in reading the 49 names. "This is an attack on humanity itself," she said in a powerful speech.